crisprcas9系统构建方法 - 豆丁网-免疫在线蚂蚁淘旗下平台-

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crisprcas9系统构建方法 - 豆丁网

来自 : 发布时间：2024-05-20

X品牌历史介绍:苏州蚂蚁淘生物科技有限公司，作为一家生命科学领域的高科技生物公司，目前代理销售二十多家欧美著名生物技术公司的产品，覆盖了免疫学、细胞生物学、分子生物学、药物筛选、生物制药、食品检测、疫苗生产等领域。

Enzymatics是马萨诸塞州贝弗利市一家快速发展的生物技术公司，已成为推动核酸检测技术的酶供应的者。Enzymatics产品价格表

货号	品名	规格	目录价	品牌
95061-250	AccuStart Taq DNA 聚合酶	250 unit/包	1170	enzymatics2018
95071-250	PerfeCTa 快速反应预混液 IQ版	250 x 20 ul reaction	2433.6	enzymatics2018
95072-250	PerfeCTa 快速反应预混液	250 x 20 ul reaction	2433.6	enzymatics2018
95073-250	PerfeCTa 快速反应预混液预装ROX版	250 x 20 ul reaction	2433.6	enzymatics2018
95074-250	PerfeCTa 快速反应预混液低ROX版	250 x 20 ul reaction	2515.5	enzymatics2018
95112-012	PerfeCTa® qPCR 核酸扩增预混试剂	10x 1.25 ml	8868.6	enzymatics2018
95141-250	AccuStart II Taq DNA 聚合酶	250 unit/包	1170	enzymatics2018
95161-025	Quanta cDNA合成预混试剂	25 reactions	1602.9	enzymatics2018
B0110	10×Blue buffer	1 ml	96.2	enzymatics2018
B0110L	10 X blue缓冲液	1.5ml/支	76.05	enzymatics2018
B0110L-200	10X Blue 缓冲液 200 ml	200 ml/瓶	7020	enzymatics2018
B0110L-40	10X Blue Buffer	40 ml/瓶	1404	enzymatics2018
B0320L-7	100 mM Magnesium Sulfate Solution	7 ml/瓶	673.4	enzymatics2018
B0330	10× Fragmentation Buffer	1 ml	96.2	enzymatics2018
B0340	Enhancer Buffer	1 ml	96.2	enzymatics2018
B0350	无海藻糖5X一步法反转定量Buffer		93.6	enzymatics2018
B0330L	10X Fragmentation Buffer	0.25 ml/支	76.05	enzymatics2018
B0340L	Enhancer buffer	0.25 ml/支	76.05	enzymatics2018
B1010L	2×Rapid Ligation Buffer	1.5 ml/支	143	enzymatics2018
B1010L-200	2×Rapid Ligation Buffer	200 ml/瓶	7020	enzymatics2018
B1010L-40	2X 快速连接缓冲液	40ml/EA	1404	enzymatics2018
B5010L-40	10 × UDG Reaction Buffer	40 ml/瓶	3848	enzymatics2018
B6030	10×T4 DNA Ligase Buffer	1 ml	96.2	enzymatics2018
B6030L	10×T4 DNA Ligase Buffer	1.5 ml/支	143	enzymatics2018
B6030L-200	10X T4 DNA 连接酶缓冲液200 ml	200 ml/瓶	7020	enzymatics2018
B6030L-40	10 X T4DNA连接酶缓冲液	40 ml/瓶	1404	enzymatics2018
B6060L-40	10×Taq连接酶反应缓冲液	40 ml	1404	enzymatics2018
B6090L-40	10 × E.coli DNA Ligation Buffer	40 ml/瓶	3848	enzymatics2018
B7020L-40	10 × φ29 DNA Polymerase Reaction Buffer	40 ml/瓶	3848	enzymatics2018
B7030	10×PCR Buffer I	1 ml	96.2	enzymatics2018
B7030L-50	10 × PCR Buffer I	50 ml/瓶	4810	enzymatics2018
B7040L-40	10 × M MuLV RT Reaction Buffer	40 ml/瓶	3848	enzymatics2018
B7102L-40	5 × VS Buffer 2	40 ml/瓶	3848	enzymatics2018
B7130L-40	5 × GC Buffer	40 ml/瓶	3848	enzymatics2018

货号：Enzymatics Y9040L品名：T4 Polynucleotide Kinase（T4多核苷酸激酶）产品描述：T4多核苷酸激酶（PNK）催化ATP的末端γ位磷酸酯转移和交换到双链和单链DNA，RNA和核苷3\\\'-单磷酸酯分子的5\\\'-羟基末端（1）。T4 PNK还表现出3\\\'-磷酸酶和2\\\'，3\\\'环磷酸二酯酶活性（2,3,4,5,6）。

规格：	10,000 U
浓度：	10,000 U/ml
保存：	-25°C至-15°C
纯度（SDS-PAGE）	> 99％
活性：	133,333U / mg
SS核酸外切酶	2000 U <5.0% released
DS核酸外切酶	2000 U <1.0% released
DS内切核酸酶	2000 U = No conversion
大肠杆菌DNA污染	2000 U <10 copies

蛋白质来源从表达重组T4多核苷酸激酶基因的大肠杆菌中纯化而得。补充：10 mM Tris-HCl50 mM KCl0.1 碌M ATP1.0 mM DTT0.1 mM EDTA50% glycerolpH 7.4 @ 25掳C随附：B9040 (10X T4 Polynucleotide Kinase Buffer)：10X多核苷酸激酶缓冲液（B9040）700mMTris-HCl100mM MgCl250mM DTTpH 7.6在25℃单位定义一个单位定义为在1X T4多核苷酸激酶反应缓冲液中，37℃下30分钟内催化掺入1 nmol [32P]（ATP供体）的酶量。质量控制分析单位表征测定单位活性使用2倍系列稀释法测量。在1X反应缓冲液中制备酶的稀释液，并将其加入到50μL含有10μMOligo dT单链DNA，1X PNK反应缓冲液和66μMATP的反应中，并将[γ-32P] ATP反应在37°孵育30分钟将其置于冰上，并使用Sambrook和Russell的方法（Molecular Cloning，v3,2001，pp.A8.25-A8.26）进行分析。蛋白质浓度（OD280）测量使用使用2.0mg / ml BSA样品（Pierce Cat＃23209）标准化的Nanodrop ND-1000分光光度计在OD280处分析2.0μL酶样品并用产品储存溶液冲切。使用消光系数63,440和分子量34,620道尔顿将观察到的3个重复样品的平均测量结果转换为mg / mL。SDS-Page（物理纯度评估）将2.0μL酶溶液加载到变性的4-20％Tris-甘氨酸SDS-PAGE凝胶上，其侧翼为宽范围MW标记和2.0μL1：100稀释的样品。电泳后，将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量，确认浓缩样品的纯度大于99％。部分产品信息：

货号

品名

规格

浓度

品牌

N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics酶产品

中文名称	ProductName	PartNo.	UnitSize
EnzScript?(MMLV逆转录酶，RNaseH-)	EnzScript?(MMLVReverseTranscriptaseRNaseH-)	P7600L	10,000U
Poly(A)聚合酶	Poly(A)Polymerase	P7460L	1,000U
核酸内切酶	RNAseH	Y9220L	5,000U
Y9220F	770U
RNA酶抑制剂	RNAseInhibitor	Y9240L	20,000U
Archer?Barcode适配体1-8，用于IonTorrent?平台,	Archer?BarcodeAdapters1-8ForIonTorrent?Platform	SA0027	N/A
Archer?Barcode适配体17-24，用于IonTorrent?平台,	Archer?BarcodeAdapters17-24ForIonTorrent?Platform	SA0029	N/A
Archer?Barcode适配体33-40，用于IonTorrent?平台,	Archer?BarcodeAdapters33-40ForIonTorrent?Platform	SA0031	N/A
Archer?Barcode适配体41-48，用于IonTorrent?平台,	Archer?BarcodeAdapters41-48ForIonTorrent?Platform	SA0032	N/A
Archer?Barcode适配体9-16，用于IonTorrent?平台,	Archer?BarcodeAdapters9-16ForIonTorrent?Platform	SA0028	N/A
Archer?FusionPlex?ARR第二代面板	Archer?FusionPlex?ARRPanelv2	AK0028-8	8reactions
Archer?FusionPlex?FGFR面板	Archer?FusionPlex?FGFRPanel	AK0030-8	8Reactions
Archer?FusionPlex?血红素面板	Archer?FusionPlex?HemePanel	AK0029-8	8reactions
Archer?FusionPlex?NTRK面板	Archer?FusionPlex?NTRKPanel	AK0031-8	8reactions
Archer?FusionPlex?肉瘤面板	Archer?FusionPlex?SarcomaPanel	AK0032-8	8reactions
Archer?MBC适配体系B，用于Illumina?	Archer?MBCAdapterSetBforIllumina?	AK0016-48	N/A
Archer?MBC适配体系C，用于Illumina?	Archer?MBCAdapterSetCforIllumina?	AK0017-48	N/A
Archer?MBC适配体系A1-A8，用于Illumina?	Archer?MBCAdaptersA1-A8forIllumina?	SA0040	N/A
Archer?MBC适配体系A17-A24，用于Illumina?	Archer?MBCAdaptersA17-A24forIllumina?	SA0042	N/A
Archer?MBC适配体系A25-A32，用于Illumina?	Archer?MBCAdaptersA25-A32forIllumina?	SA0043	N/A
Archer?MBC适配体系A33-A40，用于Illumina?	Archer?MBCAdaptersA33-A40forIllumina?	SA0044	N/A
Archer?MBC适配体系A41-A48，用于Illumina?	Archer?MBCAdaptersA41-A48forIllumina?	SA0045	N/A
Archer?MBC适配体系A9-A16，用于Illumina?	Archer?MBCAdaptersA9-A16forIllumina?	SA0041	N/A
Archer?通用RNA试剂盒，适用于Illumina?	Archer?UniversalRNAReagentKitforIllumina?	AK0024-8	N/A
Archer?通用RNA试剂盒，适用于IonTorrent?	Archer?UniversalRNAReagentKitforIonTorrent?	AK0025-8	N/A
大肠杆菌单链DNA连接蛋白	E.coliSingle-StrandedDNABindingProtein	Y9030L	1.0mg
T4噬菌体基因32编码蛋白	T4Gene32Protein	Y9130L	1.0mg
10x尿嘧啶切割系统	10xUracilCleavageSystem	Y9180L	750Reactions
大肠杆菌fpg	E.colifpg	Y9070L	4,000U
末端修复混合液（高浓度）	End-RepairMix(HighConcentration)	Y9140-HC-L	75Reactions
末端修复混合液（低浓度）	End-RepairMix(LowConcentration)	Y9140-LC-L	200Reactions
核酸内切酶VIII	EndonucleaseVIII	Y9080L	10,000U
核酸外切酶I	ExonucleaseI	X8010L	30,000U
X8010F	4,600U
核酸外切酶III	ExonucleaseIII	X8020L	50,000U
X8020F	7,700U
λ-核酸外切酶	LambdaExonuclease	X8030L	10,000U
RecA	RecA	Y9260L	1.5mg
T4DNA多聚核苷酸激酶	T4PolynucleotideKinase	Y9040L	10,000U
热分解尿嘧啶糖苷酶	ThermolabileUDG	G5020L	500U
热稳定焦磷酸酶	ThermostablePyrophosphatase	Y9370L	1,250U
Y9370F	250U
尿嘧啶DNA糖苷酶	UracilDNAGlycosylase	G5010L	10,000U
G5010F	1,540U
NGS库预备:2XVeraSeq?PCRMix–250Reactions	NGSLibraryPrep:2XVeraSeq?PCRMix–250Reactions	P7610L	250Reactions
P7610S	50Reactions
SPARK?DNA样品预备盒，用于Illumina?	SPARK?DNASamplePrepKitforIllumina?	SPK0001-V08	N/A
SPARK?DNA样品预备盒，用于IonTorrent?平台	SPARK?DNASamplePrepKitIonTorrent?Platform	SPK0002-V08	N/A
核酸10mMATP液	NucleicAcid10mMATPSolution	N2070-10-L	50μmol
核酸10mMdNTP混合液	NucleicAcid10mMdNTPSolutionMix	N2050-10-L	100μmol
N2050-10-F	20μmol
核酸100mM75μmoldNTP混合体系	NucleicAcid100mM75μmoldNTPSolutionSet	N2010L	75μmol
N2010F	20μmol
核酸100mMdATP液	NucleicAcid100mMdATPSolution	N2010-A-L	75μmol
核酸25mM100?moldNTPSolutionMix	NucleicAcid25mM100?moldNTPSolutionMix	N2050L	100?mol
N2050F	20?mol
大肠杆菌DNA连接酶	E.coliDNALigase	L6090L	2,500U
T3DNA连接酶	T3DNALigase	L6010L	900,000U
L6010F	140,000U
T4DNA连接酶	T4DNALigase	L6030-LC-L	150,000U
T4DNA连接酶（快速）	T4DNALigase(Rapid)	L6030-HC-L	240,000U
T4DNA连接酶1	T4RNALigase1	L6050L	10,000U
L6050F	1,540U
T4RNA连接酶2	T4RNALigase2	L6080L	4,500U
T4RNA连接酶2截断	T4RNALigase2Truncated	L6070L	500U
T7DNA连接酶	T7DNALigase	L6020L	900,000U
L6020F	138,000U
TaqDNA连接酶	TaqDNALigase	L6060L	20,000U
L6060F	3,100U
DNA聚合酶I	DNAPolymeraseI	P7050L	5,000U
Klenow(3′→5′exo-)（高浓度）	Klenow(3′→5′exo-)(HighConcentration)	P7010-HC-L	10,000U
P7010-LC-L	10,000U
Klenow片段	KlenowFragment	P7060L	2,500U
M-MuLV逆转录酶	M-MuLVReverseTranscriptase	P7040L	100,000U
P7040F	15,400U
MakoDNA聚合酶(3′→5′exo-)	MakoDNAPolymerase(3′→5′exo-)	P7090L	3,000U
Manta1.0DNA聚合酶(高浓度)	Manta1.0DNAPolymerase(HighConcentration)	P7140-HC-L	100,000U

苏州蚂蚁淘生物科技有限公司SuZhouebiomall BIOLOGicalTechnologyCo.,Ltd.成立于2017年，经过数年的努力已迅速成为集科研和生产为一体的专业化生物工程公司。特别是ELISA研发，代测，技术服务这一产品已使上海抚生成为中国知名公司。公司产品涵盖ELISA研发、细胞培养，各种生化试剂、各种酶类、分子生物学试剂盒、培养基、自产实验室耗材、小型仪器等。此外、上海抚生还代理了美国药典标准品，中检所标准品，sigma,ScienCell,ATCC,wak0,omega，Worthington、MBI、Bioworld、Calendon等国外各类知名公司的产品。上海抚生将进一步加强人才经营、产品经营，不断提升企业的核心竟争力，实现具有高科技产业体系、知识化创业团队的国际化的公司，服务于生命科学领域，迎接世界经济一体化所带来的机遇与挑战。

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发布于： 2024-05-20 阅读（）

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